Human CD50/ICAM3 Recombinant Protein (N-His) (HW443012)

Name: Human CD50/ICAM3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW443012
Price: 211 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HW443012
설명	Recombinant Human CD50/ICAM3 Protein, N-His (HW443012) expressed in E. coli, spanning Gly259-Val491. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	P32942
단백질 길이	Gly259-Val491
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	27.47 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3
배경	Intercellular adhesion molecule 3 (CD50/ICAM3) is a ~59 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages. Is also a ligand for integrin ITGAD/ITGB2. Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells. 1. Fawcett, J. et al. (1992) Nature 360, 481-4. PMID: 1448173 2. Vazeux, R. et al. (1992) Nature 360, 485-8. PMID: 1448174 3. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590 4. Juan, M. et al. (1993) European journal of immunology 23, 1508-12. PMID: 8325327 5. de Fougerolles, AR. et al. (1993) The Journal of experimental medicine 177, 1187-92. PMID: 8459213 6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714 7. Geijtenbeek, TB. et al. (2000) Cell 100, 575-85. PMID: 10721994
참고사항	For research use only.