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Anti-Human GAPDH Polyclonal Antibody (HY030014)

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Overview
Catalog No.HY030014
Species reactivityHuman, Mouse, Dog, Cat, Bos taurus, Columba livia, Coturnix japonica, Cricetulus griseus, Danio rerio, Chicken, Jaculus orientalis, Meleagris gallopavo, Mesocricetus auratus, Mustela putorius furo, Oryctolagus cuniculus, Ovis aries, Pelodiscus sinensis, Pongo abelii, Rat, Spermophilus citellus, Pig, Xenopus laevis
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
ClonalityPolyclonal
ImmunogenE. coli - derived recombinant Human GAPDH (Met1-Glu335).
TargetGAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase
PurificationPurified by antigen affinity column.
FormLiquid
Storage buffer0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and StorageUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
NoteFor research use only.
Images

  • Western blot

    Western blot analysis was performed using anti-GAPDH polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: PC-3 cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: MCF-7 cell lysate
    Lane 8: HT-29 cell lysate

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Anti-Human GAPDH Polyclonal Antibody

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