Anti-HIST2H2BE/H2BC21 Polyclonal Antibody (HS839014)

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Overview

Catalog No.	HS839014
Species reactivity	Human, Mouse, Rat, Anopheles gambiae, Asterias rubens, Bos taurus, Caenorhabditis elegans, Cairina moschata, Chironomus thummi thummi, Danio rerio, Drosophila erecta, Drosophila hydei, Drosophila mauritiana, Drosophila melanogaster, Drosophila orena, Drosophila sechellia, Drosophila simulans, Drosophila teissieri, Drosophila yakuba, Chicken, Holothuria tubulosa, Macaca fascicularis, Marthasterias glacialis, Oncorhynchus mykiss, Patella granatina, Patiria pectinifera, Penaeus vannamei, Platynereis dumerilii, Pongo abelii, Psammechinus miliaris, Salmo trutta, Sipunculus nudus, Strongylocentrotus purpuratus, Tigriopus californicus, Urechis caupo, Xenopus laevis, Zhangixalus schlegelii
Applications	ELISA, IHC, WB
Host species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	E. coli - derived recombinant Human HIST2H2BE/H2BC21 (Pro2-Lys126).
Target	H2B/q,Histone H2B-GL105,H2B-clustered histone 21,Histone H2B.q,H2BC21,Histone H2B type 2-E,HIST2H2BE,H2BFQ
Purification	Purified by antigen affinity column.
Accession	Q16778
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting HIST2H2BE (HS839014) at a concentration of 13.2 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of HIST2H2BE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet