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Anti-ALDOC Polyclonal Antibody (HY519014)

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Overview
Catalog No.HY519014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
ClonalityPolyclonal
ImmunogenE. coli - derived recombinant Human ALDOC (Asp34-Ser281).
TargetBrain-type aldolase, ALDOC, ALDC, Fructose-bisphosphate aldolase C
PurificationPurified by antigen affinity column.
AccessionP09972
FormLiquid
Storage buffer0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and StorageUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
NoteFor research use only.
Images

  • Immunofluorescence

    Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALDOC (HY519014) at a concentration of 24 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALDOC was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

  • Western blot

    Western blot analysis was performed using anti-ALDOC polyclonal antibody at 1μg/ml on various samples.
    Lane 1: MCF7 cell lysate
    Lane 2: LO2 cell lysate
    Lane 3: k562 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Mouse brain lysate
    Lane 6: Mouse kidney lysate
    Lane 7: Mouse muscle lysate
    Lane 8: Mouse heart lysate

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Anti-ALDOC Polyclonal Antibody

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