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Anti-CRYL1 Polyclonal Antibody (HV315014)

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Overview
Catalog No.HV315014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
ClonalityPolyclonal
ImmunogenE. coli - derived recombinant Human CRYL1 (Met1-Gln319).
TargetGul3DH, CRY, L-gulonate 3-dehydrogenase, Lambda-crystallin homolog, CRYL1
PurificationPurified by antigen affinity column.
AccessionQ9Y2S2
FormLiquid
Storage buffer0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and StorageUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
NoteFor research use only.
Images

  • Immunofluorescence

    Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CRYL1 (HV315014) at a concentration of 23.75 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CRYL1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

  • Western blot

    Western blot analysis was performed using anti-CRYL1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: MOuse liver lysate

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Anti-CRYL1 Polyclonal Antibody

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