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Anti-Human IL22RA2 Polyclonal Antibody (HV100014)

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Overview
Catalog No.HV100014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human IL22RA2 (Thr22-Pro263).
Target IL22RA2, Cytokine receptor family type 2, soluble 1, IL-22R-alpha-2, Cytokine receptor family 2 member 10, IL-22BP, CRF2-S1, Interleukin-22-binding protein, ZcytoR16, CRF2-10, Interleukin-22 receptor subunit alpha-2, IL22BP, Cytokine receptor class-II member 10, IL-22 receptor subunit alpha-2, IL-22RA2
Purification Purified by antigen affinity column.
Accession Q969J5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only.
Images
  • Immunofluorescence

    HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting IL22RA2 (HV100014) at a concentration of 29.2 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of IL22RA2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

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