











| Catalog No. | HY095010 | 
|---|---|
| Species reactivity | Human | 
| Applications | ELISA, FCM, Neutralization | 
| Host species | Human | 
| Isotype | IgG1, lambda | 
| Clone ID | Iv0063 | 
| Clonality | Monoclonal | 
| Target | IGF-I, IGF1, Mechano growth factor, MGF, Insulin-like growth factor I, IBP1, Somatomedin-C | 
| Endotoxin level | Please contact with the lab for this information. | 
| Purity | >95% as determined by SDS-PAGE. | 
| Purification | Protein A/G purified from cell culture supernatant. | 
| Accession | P05019 | 
| Form | Liquid | 
| Storage buffer | 0.01M PBS, pH 7.4.                                                 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.  | 
                                        
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | 
| Note | For research use only. Not suitable for clinical or therapeutic use. | 

SEC-HPLC detection for InVivoMAb Anti-Human IGF1 (ABV0025).

Flow-cytometry using anti-human IGF2 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF2 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IGF1 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF1 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IGF2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF2 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IGF1 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF1 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human IGF1 (Iv0063).




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