Catalog No. | HY095010 |
---|---|
Species reactivity | Human |
Applications | ELISA, FCM, Neutralization |
Host species | Human |
Isotype | IgG1, lambda |
Clone ID | Iv0063 |
Clonality | Monoclonal |
Target | IGF-I, IGF1, Mechano growth factor, MGF, Insulin-like growth factor I, IBP1, Somatomedin-C |
Endotoxin level | Please contact with the lab for this information. |
Purity | >95% as determined by SDS-PAGE. |
Purification | Protein A/G purified from cell culture supernatant. |
Accession | P05019 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
Note | For research use only. Not suitable for clinical or therapeutic use. |
SEC-HPLC detection for InVivoMAb Anti-Human IGF1 (ABV0025).
Flow-cytometry using anti-human IGF2 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF2 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human IGF1 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF1 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human IGF2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF2 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human IGF1 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF1 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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