Anti-NME6 Polyclonal Antibody (HF556014)

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HF556014

Species reactivity

Mouse, Rat, Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NME6 (Leu11-Ala186).

Target

nm23-H6, Nucleoside diphosphate kinase 6, IPIA-alpha, NME6, NDP kinase 6, Inhibitor of p53-induced apoptosis-alpha, NDK 6

Purification

Purified by antigen affinity column.

Accession

O75414

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting NME6 (HF556014) at a concentration of 26.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of NME6 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-NME6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Lncap cell lysate
Lane 2: U20S cell lysate

References

Datasheet

Anti-NME6 Polyclonal Antibody.pdf

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Anti-NME6 Polyclonal Antibody (HF556014)

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