Anti-S100A11 Polyclonal Antibody (HB147014)

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Overview

Catalog No.	HB147014
Species reactivity	Human, Mouse, Rat, Oryctolagus cuniculus
Applications	ELISA, IHC, WB
Host species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	E. coli - derived recombinant Human S100A11 (Met1-Thr105).
Target	MLN70,Calgizzarin,S100C,MLN 70,Protein S100-C,S100A11,S100 calcium-binding protein A11,Protein S100-A11,Metastatic lymph node gene 70 protein
Purification	Purified by antigen affinity column.
Accession	P31949
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting S100A11 (HB147014) at a concentration of 27 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of S100A11 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet