Anti-Human CD3E Antibody (UCHT-1) (HY057207)

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Overview

Catalog No.	HY057207
Species reactivity	Human
Applications	FCM
Host species	Rabbit
Isotype	IgG, kappa
Clone ID	UCHT-1
Clonality	Monoclonal
Target	T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain，UCHT1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P07766
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human CD3E antibody.CD3E Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # HY057207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD3E antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # HY057207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD3E antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # HY057207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet