Anti-Human EGFR & HGFR/c-MET Bispecific Reference Antibody (Amivantamab, RUO) (HF004076)

Name: Anti-Human EGFR & HGFR/c-MET Bispecific Reference Antibody (Amivantamab, RUO)
Brand: abinScience
SKU: HF004076
Price: 176 USD
Availability: InStock

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Overview

Catalog No.	HF004076
Description	Amivantamab (HF004076) is a research-grade recombinant antibody targeting HER1. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Expression system	Mammalian cells
Clonality	Monoclonal
Tested applications	FCM
Target	HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB, Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET
Endotoxin level	< 10 EU/mg
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P00533 & P08581
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Bispecific, JNJ-61186372, JNJ-6372, CNTO-4424, JNJ61186372, JNJ-611, 2171511-58-1
Background	Amivantamab (JNJ-61186372) is a human EGFR-MET bispecific antibody with immune anticancer activity. Amivantamab inhibits ligand binding, promotes endocytosis and degradation of receptor-antibody complexes, and induces Fc-dependent cytokinesis in macrophages and antibody-dependent cytotoxicity in natural killer cells.
Note	For research use only. Not suitable for clinical or therapeutic use.

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SDS-PAGE

SDS-PAGE for Research Grade Amivantamab.
SEC-HPLC

SEC-HPLC detection for Research Grade Amivantamab.
Bioactivity

Detects EGFR/ERBB1/HER1 in indirect ELISAs.
Bioactivity

Detects recombinant human Met (Cat No.: HY196021) in indirect ELISA.
Flow CytoMetry

Flow-cytometry using APC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human EGFR monoclonal antibody (Catalog: HF004076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR monoclonal antibody (Catalog: HF004076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: HF004076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human EGFR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFR monoclonal antibody (Catalog: HF004076) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human EGFR & HGFR/c-MET Bispecific Reference Antibody (Amivantamab, RUO).pdf

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Anti-Human EGFR & HGFR/c-MET Bispecific Reference Antibody (Amivantamab, RUO) (HF004076)

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References

Datasheet

SDS

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