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Research Grade Abituzumab (HY065016)

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  • 100ug
  • 1mg
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  • Datasheet
Overview
Catalog No.HY065016
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHumanized
IsotypeIgG2-kappa
Expression systemMammalian Cells
ClonalityMonoclonal
TargetMSK8, VNRA, Vitronectin receptor, Integrin alpha-V, Vitronectin receptor subunit alpha, CD51, ITGAV, VTNR
Endotoxin levelPlease contact the lab for this information.
Purity>95% purity as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionP06756
FormLiquid
Storage buffer0.01M PBS, pH 7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate NamesDI-17E6, EMD525797, 1105038-73-0
BackgroundAbituzumab (DI17E6) is a humanised anti-integrin αV monoclonal antibody (IgG2 type). Abituzumab effectively reduces the phosphorylation of FAK, Akt and ERK. Abituzumab can be used in cancer research, particularly in prostate cancer.
NoteFor research use only. Not suitable for clinical or therapeutic use.
Images
  • Bioactivity

    SEC-HPLC detection for Research Grade Abituzumab.

  • Flow Cytometry

    Flow-cytometry using anti-human CD51 antibody. A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD51 monoclonal antibody (Catalog HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Research Grade Abituzumab.

  • Flow CytoMetry

    Flow-cytometry using APC anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using PE anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References

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