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Anti-CACYBP Polyclonal Antibody (HV095014)

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Overview
Catalog No.HV095014
Species reactivityHuman, Mouse, Rat, Bos taurus, Macaca fascicularis, Pongo abelii
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CACYBP (Ala2-Phe228).
Target S100A6-binding protein, SIP, Siah-interacting protein, CacyBP, CACYBP, hCacyBP, S100A6BP, Calcyclin-binding protein
Purification Purified by antigen affinity column.
Accession Q9HB71
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only.
Images
  • Immunofluorescence

    Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CACYBP (HV095014) at a concentration of 14.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CACYBP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

  • Western blot

    Western blot analysis was performed using anti-CACVBP polyclonal antibody at 1μg/ml on various samples.
    Lane 1: K562 cell lysate
    Lane 2: HCT116 cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: HL60 cell lysate
    Lane 7: Jurkat cell lysate
    Lane 8: Rat brain lysate
    Lane 9: Rat liver lysate

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