Catalog No. | HV095014 | ||||||||
---|---|---|---|---|---|---|---|---|---|
Species reactivity | Human, Mouse, Rat, Bos taurus, Macaca fascicularis, Pongo abelii | ||||||||
Applications | ELISA, IHC, WB | ||||||||
Host species | Rabbit | ||||||||
Isotype | IgG | ||||||||
Clonality | Polyclonal | ||||||||
Immunogen | E. coli - derived recombinant Human CACYBP (Ala2-Phe228). | ||||||||
Target | S100A6-binding protein, SIP, Siah-interacting protein, CacyBP, CACYBP, hCacyBP, S100A6BP, Calcyclin-binding protein | ||||||||
Purification | Purified by antigen affinity column. | ||||||||
Accession | Q9HB71 | ||||||||
Form | Liquid | ||||||||
Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. | ||||||||
Product Usage Information |
| ||||||||
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
Note | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CACYBP (HV095014) at a concentration of 14.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CACYBP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-CACVBP polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: HCT116 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: 293T cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HL60 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Rat brain lysate
Lane 9: Rat liver lysate
+86-027-65523339
Building C, No. 666, Shen Dun Si Lu, Wuhan, 430206, China