Anti-CD326/EPCAM Polyclonal Antibody (HB104014)

Name: Anti-CD326/EPCAM Polyclonal Antibody
Brand: abinScience
SKU: HB104014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HB104014

Species reactivity

Human, Macaca mulatta

Applications

ELISA, IHC, WB, IF

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD326/EPCAM (Gln24-Lys265).

Target

Epithelial glycoprotein 314, hEGP314, Major gastrointestinal tumor-associated protein GA733-2, EGP314, TACSTD1, Adenocarcinoma-associated antigen, TROP1, EPCAM, Epithelial glycoprotein, KS 1/4 antigen, Tumor-associated calcium signal transducer 1, M4S1, CD326, Ep-CAM, EGP, Epithelial cell adhesion molecule, M1S2, KSA, Cell surface glycoprotein Trop-1, GA733-2, Epithelial cell surface antigen, MIC18

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P16422

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting EpCAM (HB104014) at a concentration of 20.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of EpCAM was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-EPCAM polyclonal antibody at 1μg/ml on various samples.
Lane 1: HCT116 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Mouse colon lysate

References

Datasheet

Anti-CD326/EPCAM Polyclonal Antibody.pdf

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD326/EPCAM Polyclonal Antibody (HB104014)

Overview

Images

References

Datasheet

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