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Anti-Human BAX Antibody (6A7) (HC491013)

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  • Overview
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  • References
  • Datasheet
Overview
Catalog No.HC491013
Species reactivityHuman, Mouse
ApplicationsELISA, FCM, WB
Host speciesMouse
IsotypeIgG1, kappa
Clone ID6A7
ClonalityMonoclonal
TargetBAX, Bcl2-L-4, BCL2L4, Bcl-2-like protein 4, Apoptosis regulator BAX
Endotoxin levelPlease contact with the lab for this information.
Purity>95% as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionQ07812
FormLiquid
Storage buffer0.01M PBS, pH 7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
NoteFor research use only.
Images

  • Bioactivity

    SEC-HPLC detection for Anti-Human BAX Antibody (6A7).

  • Flow Cytometry

    Flow-cytometry using anti-human BAX antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human BAX antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human BAX antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human BAX Antibody (6A7).

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using PE anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

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Anti-Human BAX Antibody (6A7)

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