Anti-Human CD22 Antibody (RFB-4) (HB011307)

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Overview

Catalog No.	HB011307
Species reactivity	Human
Applications	ELISA, FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	RFB-4
Clonality	Monoclonal
Target	Sialic acid-binding Ig-like lectin 2, Siglec-2, CD22, T-cell surface antigen Leu-14, BL-CAM, SIGLEC2, B-lymphocyte cell adhesion molecule, B-cell receptor CD22, RFB4, RFB 4
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P20273
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

Detects CD22 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD22 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD22 antibody monoclonal antibody (Catalog # HB011307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet