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Anti-Human CD33 Antibody (SAA1404) (HB185307)

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Overview
Catalog No.HB185307
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA1404
Clonality Monoclonal
Target Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P20138
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images

  • Bioactivity

    SEC-HPLC detection for Anti-Human CD33 Antibody (ABS0480).

  • Flow Cytometry

    Flow-cytometry using anti-human CD33 antibody.CD33 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD33 antibody.RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human CD33 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD33 monoclonal antibody (Catalog: HB185307) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human CD33 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 monoclonal antibody (Catalog: HB185307, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Anti-Human CD33 Antibody (SAA1404)

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