









| Catalog No. | HY339010 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, FCM, Neutralization |
| Host species | Mouse |
| Isotype | IgG2a, kappa |
| Clone ID | Iv0120 |
| Clonality | Monoclonal |
| Target | Liver-type arginase, ARG1, Type I arginase, Arginase-1 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P05089 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. Not suitable for clinical or therapeutic use. |

Detects Human ARG1/Arginase-1 in indirect ELISAs.

Flow-cytometry using anti-human Arginase 1 antibody.M2 macrophages derived from THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # HY339010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human Arginase 1 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # HY339010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120).

Flow-cytometry using PE anti-human Arginase 1 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human Arginase 1 monoclonal antibody (Catalog: HY339010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.




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