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Anti-ARG1/Arginase-1 Polyclonal Antibody (HY339024)

Anti-ARG1/Arginase-1 Polyclonal Antibody
Anti-ARG1/Arginase-1 Polyclonal Antibody
Anti-ARG1/Arginase-1 Polyclonal Antibody
Anti-ARG1/Arginase-1 Polyclonal Antibody
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  • Overview

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Overview
Catalog No.HY339024
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ARG1/Arginase-1 (Glu44-Lys322).
Target Liver-type arginase, ARG1, Type I arginase, Arginase-1
Purification Purified by antigen affinity column.
Accession P05089
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Arginase-1 (ARG1) is a ~34 kDa protein. Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.

Note For research use only.
Images
  • Anti-ARG1/Arginase-1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ARG1/Arginase-1 polyclonal antibody at 1ug/mL on recombinant ARG1/Arginase-1 (Catalog No. HY339022).

  • Anti-ARG1/Arginase-1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ARG1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Rat liver lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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