Anti-Human CD33 Antibody (P67.6), PE (HB185127)

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Overview

Catalog No.	HB185127
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG4, kappa
Clone ID	P67.6
Conjugation	PE
Clonality	Monoclonal
Target	Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3
Endotoxin level	Please contact with the lab for this information.
Accession	P20138
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow Cytometry

Flow-cytometry using PE anti-human CD33 antibody. RMPI-8226 cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-human CD33 antibody monoclonal antibody (Catalog # HB185127, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using PE anti-human CD33 antibody. HL-60 cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-human CD33 antibody monoclonal antibody (Catalog # HB185127, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet