Recombinant Human UBE2S Protein, N-His (HS820012)

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Catalog No.	HS820012
Description	Recombinant Human UBE2S Protein, N-His (HS820012) expressed in E. coli, spanning Met1-Ala209. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q16763
Protein length	Met1-Ala209
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ubiquitin-protein ligase S, E2EPF, Ubiquitin-conjugating enzyme E2 S, E2-EPF, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-conjugating enzyme E2-24 kDa, E2 ubiquitin-conjugating enzyme S, Ubiquitin carrier protein S, UBE2S
Background	Ubiquitin-conjugating enzyme E2 S (UBE2S) is a ~23 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. 1. Garnett, MJ. et al. (2009) Nature cell biology 11, 1363-9. PMID: 19820702 2. Williamson, A. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 18213-8. PMID: 19822757 3. Sheng, Y. et al. (2012) Molecular & cellular proteomics : MCP 11, 329-41. PMID: 22496338 4. Brown, NG. et al. (2016) Cell 165, 1440-1453. PMID: 27259151 5. Kuang, P. et al. (2016) Scientific reports 6, 37441. PMID: 27910872 7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386 8. Bremm, A. et al. (2010) Nature structural & molecular biology 17, 939-47. PMID: 20622874
Note	For research use only.

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SDS-PAGE for Recombinant Human UBE2S Protein, N-His

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Datasheet

Recombinant Human UBE2S Protein, N-His.pdf

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Recombinant Human UBE2S Protein, N-His (HS820012)

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