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Anti-Biotin Recombinant Antibody (M33), HRP (YP709913)

Anti-Biotin Recombinant Antibody (M33), HRP
Anti-Biotin Recombinant Antibody (M33), HRP
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Overview
Catalog No.YP709913
Species reactivityGeneral
ApplicationsELISA, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDM33
Clonality Monoclonal
Target Biotin, Vitamin B7, Vitamin H, BTN
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
References 1. Zempleni, et al. Biotin biochemistry and human requirements. J Nutr Biochem, 1999. 10(3): p. 128-38. [YP709913]
2. Mock, D.M.. Biotin status: which are valid indicators and how do we know? J Nutr, 1999. 129(2S Suppl): p. 498S-503S. [YP709913]
3. Zempleni J, et al. Biotin. Biofactors. 2009 Jan-Feb;35(1):36-46. [YP709913]
4. Vadlapudi AD, et al. Biotin uptake by T47D breast cancer cells: functional and molecular evidence of sodium-dependent multivitamin transporter (SMVT). Int J Pharm. 2013 Jan 30;441(1-2):535-43. [YP709913]
5. Sghaier R, et al. Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes. Free Radic Res. 2019 May;53(5):535-561. [YP709913]
6. Aldahmash BA, et al. Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice. Saudi J Biol Sci. 2015 Sep;22(5):564-9. [YP709913]
7. He P, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2020 Feb;97:216-234. View More [YP709913]
Note For research use only.
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  • Anti-Biotin Recombinant Antibody (M33), HRP

    SDS-PAGE

    SDS-PAGE for Anti-Biotin Antibody (M33), HRP.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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