Anti-CDK6 Polyclonal Antibody (HX252014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HX252014

Description

Anti-CDK6 Polyclonal Antibody (HX252014) is a rabbit polyclonal antibody detecting CDK6 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CDK6 (Met1-Ala326).

Target

Cell division protein kinase 6, Cyclin-dependent kinase 6, Serine/threonine-protein kinase PLSTIRE, CDKN6, CDK6

Purification

Purified by antigen affinity column.

Accession

Q00534

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cyclin-dependent kinase 6 (CDK6) is a ~36 kDa protein. Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and negatively regulates cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells).

1. Hussain, MS. et al. (2013) Human molecular genetics 22, 5199-214. PMID: 23918663

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CDK6 polyclonal antibody at 1ug/mL on recombinant CDK6 (Catalog No. HX252012).

References

Datasheet

Anti-CDK6 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CDK6 Polyclonal Antibody (HX252014)

Overview

Images

References

Datasheet

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