Anti-QPCT Polyclonal Antibody (HS786014)

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50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

HS786014

Description

Anti-QPCT Polyclonal Antibody (HS786014) is a rabbit polyclonal antibody detecting QPCT in ELISA, IHC, WB. Suitable for Bovine and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Bos taurus, Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human QPCT (Val29-Leu361).

Target

sQC, Glutaminyl-tRNA cyclotransferase, QC, QPCT, Glutamyl cyclase, EC, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase

Purification

Purified by antigen affinity column.

Accession

Q16769

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glutaminyl-peptide cyclotransferase (QPCT) is a ~40 kDa protein. Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-amyloid-beta. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-QPCT polyclonal antibody at 1ug/mL on recombinant QPCT (Catalog No. HS786012).

References

Datasheet

Anti-QPCT Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-QPCT Polyclonal Antibody (HS786014)

Overview

Images

References

Datasheet

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