Anti-ZC3H12A Polyclonal Antibody (HS805014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HS805014

Description

Anti-ZC3H12A Polyclonal Antibody (HS805014) is a rabbit polyclonal antibody detecting ZC3H12A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ZC3H12A (Asp426-Glu599).

Target

Regnase-1, MCPIP, Endoribonuclease ZC3H12A, Reg1, MCPIP-1, MCP-induced protein 1, ZC3H12A, Zinc finger CCCH domain-containing protein 12A, MCPIP1, Monocyte chemotactic protein-induced protein 1

Purification

Purified by antigen affinity column.

Accession

Q5D1E8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Endoribonuclease ZC3H12A is a ~65 kDa protein. Endoribonuclease involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay. Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation. Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL). Inhibits cooperatively with ZC3H12A the differentiation of helper T cells Th17 in lungs.

1. Mizgalska, D. et al. (2009) The FEBS journal 276, 7386-99. PMID: 19909337

2. Garg, AV. et al. (2015) Immunity 43, 475-87. PMID: 26320658

3. Xu, J. et al. (2012) Nucleic acids research 40, 6957-65. PMID: 22561375

4. Huang, S. et al. (2015) The Journal of biological chemistry 290, 20782-20792. PMID: 26134560

5. Suzuki, HI. et al. (2011) Molecular cell 44, 424-36. PMID: 22055188

6. Roy, A. et al. (2013) American journal of physiology. Cell physiology 305, C1021-32. PMID: 24048733

7. Wang, W. et al. (2015) The Journal of biological chemistry 290, 13372-85. PMID: 25861989

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ZC3H12A polyclonal antibody at 1ug/mL on recombinant ZC3H12A (Catalog No. HS805012).

References

Datasheet

Anti-ZC3H12A Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ZC3H12A Polyclonal Antibody (HS805014)

Overview

Images

References

Datasheet

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