Anti-FN3K Polyclonal Antibody (HV281014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HV281014

Description

Anti-FN3K Polyclonal Antibody (HV281014) is a rabbit polyclonal antibody detecting FN3K in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FN3K (Met1-Lys309).

Target

Protein-ribulosamine 3-kinase FN3K, Protein-psicosamine 3-kinase FN3K, FN3K, Fructosamine-3-kinase

Purification

Purified by antigen affinity column.

Accession

Q9H479

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Fructosamine-3-kinase (FN3K) is a ~35 kDa protein. Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate. Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions. Involved in intracellular deglycation in erythrocytes. Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function. Also able to phosphorylate psicosamines and ribulosamines.

1. Delpierre, G. et al. (2000) Diabetes 49, 1627-34. PMID: 11016445

2. Szwergold, BS. et al. (2001) Diabetes 50, 2139-47. PMID: 11522682

3. Delpierre, G. et al. (2002) The Biochemical journal 365, 801-8. PMID: 11975663

4. Collard, F. et al. (2003) Diabetes 52, 2888-95. PMID: 14633848

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FN3K polyclonal antibody at 1ug/mL on recombinant FN3K (Catalog No. HV281012).

References

Datasheet

Anti-FN3K Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-FN3K Polyclonal Antibody (HV281014)

Overview

Images

References

Datasheet

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