Anti-Bacillus subtilis ffp/sfp Polyclonal Antibody (JN862014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

JN862014

Description

Anti-Bacillus subtilis ffp/sfp Polyclonal Antibody (JN862014) is a rabbit polyclonal antibody detecting ffp in ELISA, IHC, WB. Suitable for Bacillus subtilis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Bacillus subtilis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Bacillus subtilis ffp/sfp (Met1-Leu224).

Target

4'-phosphopantetheinyl transferase ffp, 2.7.8.-, Fengycin synthase-activating enzyme, ffp, sfp

Purification

Purified by antigen affinity column.

Accession

Q9F4F7

RRID

Anti-Bacillus subtilis ffp/sfp Polyclonal Antibody (abinScience Cat# JN862014, RRID:AB_3730415)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

4'-phosphopantetheinyl transferase ffp (sfp) is a ~25 kDa protein. May activate the peptidyl carrier protein (PCP) domains of fengycin synthase by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Bacillus subtilis ffp/sfp polyclonal antibody at 1ug/mL on recombinant Bacillus subtilis ffp/sfp (Catalog No. JN862012).

References

Datasheet

Anti-Bacillus subtilis ffp/sfp Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Bacillus subtilis ffp/sfp Polyclonal Antibody (JN862014)

Overview

Images

References

Datasheet

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