Anti-Human EGLN2/EIT6 Polyclonal Antibody (HP528014)

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50ug
100ug

Number:

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Datasheet

Overview

Catalog No.

HP528014

Description

Anti-Human EGLN2/EIT6 Polyclonal Antibody (HP528014) is a rabbit polyclonal antibody detecting EGLN2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EGLN2/EIT6 (Glu167-Thr407).

Target

Estrogen-induced tag 6, EIT-6, HIF-prolyl hydroxylase 1, HIF-PH1, HPH-3, HPH-1, EIT6, PHD1, Prolyl hydroxylase domain-containing protein 1, Egl nine homolog 2, Prolyl hydroxylase EGLN2, Hypoxia-inducible factor prolyl hydroxylase 1, EGLN2

Purification

Purified by antigen affinity column.

Accession

Q96KS0

RRID

Anti-Human EGLN2/EIT6 Polyclonal Antibody (abinScience Cat# HP528014, RRID:AB_3730444)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Prolyl hydroxylase EGLN2 (EIT6) is a ~43 kDa protein. Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A.

1. Epstein, AC. et al. (2001) Cell 107, 43-54. PMID: 11595184

2. McNeill, LA. et al. (2002) Bioorganic & medicinal chemistry letters 12, 1547-50. PMID: 12039559

3. Cobb, RR. et al. (2005) Protein expression and purification 42, 295-304. PMID: 15925519

4. Tian, YM. et al. (2006) The Biochemical journal 397, 179-86. PMID: 16509823

5. Cummins, EP. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 18154-9. PMID: 17114296

6. Moser, SC. et al. (2013) Developmental cell 26, 381-92. PMID: 23932902

7. Huang, J. et al. (2002) The Journal of biological chemistry 277, 39792-800. PMID: 12181324

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human EGLN2/EIT6 polyclonal antibody at 1ug/mL on recombinant Human EGLN2/EIT6 (Catalog No. HP528012).

References

Datasheet

Anti-Human EGLN2/EIT6 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EGLN2/EIT6 Polyclonal Antibody (HP528014)

Overview

Images

References

Datasheet

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