Anti-MFN2 Polyclonal Antibody (HF769014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HF769014

Description

Anti-MFN2 Polyclonal Antibody (HF769014) is a rabbit polyclonal antibody detecting MFN2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MFN2 (His20-Arg400).

Target

Transmembrane GTPase MFN2, Mitofusin-2, KIAA0214, CPRP1, MFN2

Purification

Purified by antigen affinity column.

Accession

O95140

RRID

Anti-MFN2 Polyclonal Antibody (abinScience Cat# HF769014, RRID:AB_3730470)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitofusin-2 (MFN2) is a ~86 kDa protein. Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes.

1. Santel, A. et al. (2001) Journal of cell science 114, 867-74. PMID: 11181170

2. Rojo, M. et al. (2002) Journal of cell science 115, 1663-74. PMID: 11950885

3. Casasnovas, C. et al. (2010) Journal of medical genetics 47, 249-56. PMID: 19889647

4. Senyilmaz, D. et al. (2015) Nature 525, 124-8. PMID: 26214738

5. Cao, YL. et al. (2017) Nature 542, 372-376. PMID: 28114303

6. Chen, Y. et al. (2013) Science (New York, N.Y.) 340, 471-5. PMID: 23620051

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MFN2 polyclonal antibody at 1ug/mL on recombinant MFN2 (Catalog No. HF769012).

References

Datasheet

Anti-MFN2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MFN2 Polyclonal Antibody (HF769014)

Overview

Images

References

Datasheet

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