Anti-Human LIN28A Polyclonal Antibody (HP172014)

Name: Anti-Human LIN28A Polyclonal Antibody
Brand: abinScience
SKU: HP172014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
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Overview

Catalog No.

HP172014

Description

Anti-Human LIN28A Polyclonal Antibody (HP172014) is a rabbit polyclonal antibody detecting LIN28A in ELISA, IHC, WB. Suitable for Human, Mouse, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Chicken

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LIN28A (Gln36-Gly111).

Target

CSDD1, Zinc finger CCHC domain-containing protein 1, LIN28, LIN28A, Lin-28A, Protein lin-28 homolog A, ZCCHC1

Purification

Purified by antigen affinity column.

Accession

Q9H9Z2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein lin-28 homolog A (LIN28A) is a ~22 kDa protein. RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA.

1. Jin, J. et al. (2011) Nucleic acids research 39, 3724-34. PMID: 21247876

2. Heo, I. et al. (2008) Molecular cell 32, 276-84. PMID: 18951094

3. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396

4. Piskounova, E. et al. (2011) Cell 147, 1066-79. PMID: 22118463

5. Thornton, JE. et al. (2012) RNA (New York, N.Y.) 18, 1875-85. PMID: 22898984

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human LIN28A polyclonal antibody at 1ug/mL on recombinant Human LIN28A (Catalog No. HP172012).

References

Datasheet

Anti-Human LIN28A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human LIN28A Polyclonal Antibody (HP172014)

Overview

Images

References

Datasheet

SDS

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