Anti-Human WDR1 Polyclonal Antibody (HF897024)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HF897024

Description

Anti-Human WDR1 Polyclonal Antibody (HF897024) is a rabbit polyclonal antibody detecting WDR1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Chicken, Xenopus laevis, Xenopus tropicalis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WDR1 (Met1-Lys317).

Target

WD repeat-containing protein 1, Actin-interacting protein 1, WDR1, AIP1, NORI-1

Purification

Purified by antigen affinity column.

Accession

O75083

RRID

Anti-Human WDR1 Polyclonal Antibody (abinScience Cat# HF897024, RRID:AB_3730646)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

WD repeat-containing protein 1 (WDR1) is a ~66 kDa protein. Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing. Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions. Involved in myocardium sarcomere organization.

1. Fujibuchi, T. et al. (2005) Biochemical and biophysical research communications 327, 268-75. PMID: 15629458

2. Kuhns, DB. et al. (2016) Blood 128, 2135-2143. PMID: 27557945

3. Pfajfer, L. et al. (2018) The Journal of allergy and clinical immunology 142, 1589-1604.e11. PMID: 29751004

4. Kato, A. et al. (2008) The Biochemical journal 414, 261-70. PMID: 18494608

5. Lechuga, S. et al. (2015) American journal of physiology. Gastrointestinal and liver physiology 308, G745-56. PMID: 25792565

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human WDR1 polyclonal antibody at 1ug/mL on recombinant Human WDR1 (Catalog No. HF897022).

References

Datasheet