Anti-Human GALE Polyclonal Antibody (HC414014)

Name: Anti-Human GALE Polyclonal Antibody
Brand: abinScience
SKU: HC414014
Price: 96 USD
Availability: InStock

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20ug
50ug
100ug

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Overview
Images
References
Datasheet

Overview

Catalog No.

HC414014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GALE (Met1-Ala348).

Target

UDP-galactose 4-epimerase, UDP-N-acetylgalactosamine 4-epimerase, Galactowaldenase, UDP-glucose 4-epimerase, GALE, UDP-GalNAc 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-GlcNAc 4-epimerase

Purification

Purified by antigen affinity column.

Accession

Q14376

RRID

Anti-Human GALE Polyclonal Antibody (abinScience Cat# HC414014, RRID:AB_3730914)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

UDP-glucose 4-epimerase (GALE) is a ~38 kDa protein. Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GALE polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate

References

Datasheet

Anti-Human GALE Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GALE Polyclonal Antibody (HC414014)

Overview

Images

References

Datasheet

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