Anti-Human GNAO1 Polyclonal Antibody (HY153014)

Name: Anti-Human GNAO1 Polyclonal Antibody
Brand: abinScience
SKU: HY153014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): $

Spec:

20ug
50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HY153014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GNAO1 (Met1-Tyr354).

Target

Guanine nucleotide-binding protein G(o) subunit alpha, GNAO1

Purification

Purified by antigen affinity column.

Accession

P09471

RRID

Anti-Human GNAO1 Polyclonal Antibody (abinScience Cat# HY153014, RRID:AB_3730915)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Guanine nucleotide-binding protein G(o) subunit alpha (GNAO1) is a ~40 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins.

1. García-Nafría, J. et al. (2018) Nature 558, 620-623. PMID: 29925951

2. Ping, YQ. et al. (2021) Nature 589, 620-626. PMID: 33408414

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GNAO1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse cerebellum lysate
Lane 2: Rat brain lysate
Lane 3: Rat cerebellum lysate

References

Datasheet

Anti-Human GNAO1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GNAO1 Polyclonal Antibody (HY153014)

Overview

Images

References

Datasheet

Quick access

Contact Us