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Anti-Human TWIST2 Polyclonal Antibody (HP431014)

Anti-Human TWIST2 Polyclonal Antibody
Anti-Human TWIST2 Polyclonal Antibody
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Overview
Catalog No.HP431014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TWIST2 (Ser60-His160).
Target Class A basic helix-loop-helix protein 39, Twist-related protein 2, DERMO1, Dermo-1, BHLHA39, TWIST2, bHLHa39, Dermis-expressed protein 1
Purification Purified by antigen affinity column.
Accession Q8WVJ9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Twist-related protein 2 (TWIST2) is a ~18 kDa protein. Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.

Note For research use only.
Images
  • Anti-Human TWIST2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Human TWIST2 polyclonal antibody at 1ug/mL on recombinant Human TWIST2 (Catalog No. HP431012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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