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Anti-NOTCH4 Polyclonal Antibody (HV159014)

Anti-NOTCH4 Polyclonal Antibody
Anti-NOTCH4 Polyclonal Antibody
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  • 100ug
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  • Datasheet

Overview
Catalog No.HV159014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NOTCH4 (Asp1715-Lys2002).
Target NOTCH4, INT3, hNotch4, Neurogenic locus notch homolog protein 4, Notch 4
Purification Purified by antigen affinity column.
Accession Q99466
RRID Anti-NOTCH4 Polyclonal Antibody (abinScience Cat# HV159014, RRID:AB_3722809)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Neurogenic locus notch homolog protein 4 (NOTCH4) is a ~209 kDa protein. Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.

Note For research use only.
Images
  • Anti-NOTCH4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NOTCH4 polyclonal antibody at 1ug/mL on recombinant NOTCH4 (Catalog No. HV159012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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