Anti-Clostridium perfringens plc/Phospholipase C/Alpha-toxin Polyclonal Antibody (JN878014)

Name: Anti-Clostridium perfringens plc/Phospholipase C/Alpha-toxin Polyclonal Antibody
Brand: abinScience
SKU: JN878014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

JN878014

Species reactivity

Clostridium perfringens

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Clostridium perfringens plc/Phospholipase C/Alpha-toxin (Ala28-Lys398).

Target

Phospholipase C, PLC, 3.1.4.3, Alpha-toxin, Hemolysin, Lecithinase, Phosphatidylcholine cholinephosphohydrolase, plc, cpa, CPE0036

Purification

Purified by antigen affinity column.

Accession

P0C216

RRID

Anti-Clostridium perfringens plc/Phospholipase C/Alpha-toxin Polyclonal Antibody (abinScience Cat# JN878014, RRID:AB_3722923)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phospholipase C (plc/Alpha-toxin) is a ~45 kDa protein. Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Clostridium perfringens plc/Phospholipase C/Alpha-toxin polyclonal antibody at 1ug/mL on recombinant Clostridium perfringens plc/Phospholipase C/Alpha-toxin (Catalog No. JN878012).

References

Datasheet

Anti-Clostridium perfringens plc/Phospholipase C/Alpha-toxin Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Clostridium perfringens plc/Phospholipase C/Alpha-toxin Polyclonal Antibody (JN878014)

Overview

Images

References

Datasheet

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