Anti-Human MBD1 Polyclonal Antibody (HV136014)

Name: Anti-Human MBD1 Polyclonal Antibody
Brand: abinScience
SKU: HV136014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HV136014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MBD1 (Met1-Leu216).

Target

CXXC3, PCM1, CXXC-type zinc finger protein 3, Protein containing methyl-CpG-binding domain 1, Methyl-CpG-binding domain protein 1, MBD1, Methyl-CpG-binding protein MBD1

Purification

Purified by antigen affinity column.

Accession

Q9UIS9

RRID

Anti-Human MBD1 Polyclonal Antibody (abinScience Cat# HV136014, RRID:AB_3722962)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Methyl-CpG-binding domain protein 1 (MBD1) is a ~66 kDa protein. Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human MBD1 polyclonal antibody at 1ug/mL on recombinant Human MBD1 (Catalog No. HV136012).

References

Datasheet

Anti-Human MBD1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MBD1 Polyclonal Antibody (HV136014)

Overview

Images

References

Datasheet

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