Anti-CUBN Polyclonal Antibody (HT153014)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HT153014

Description

Anti-CUBN Polyclonal Antibody (HT153014) is a rabbit polyclonal antibody detecting CUBN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CUBN (Cys3511-Ser3623).

Target

Cubilin, 460 kDa receptor, Intrinsic factor-vitamin B12 receptor, IFCR, Intestinal intrinsic factor receptor, Intrinsic factor-cobalamin receptor, CUBN

Purification

Purified by antigen affinity column.

Accession

O60494

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cubilin (CUBN) is a ~398 kDa protein. Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium.

1. Kozyraki, R. et al. (1999) Nature medicine 5, 656-61. PMID: 10371504

2. Kozyraki, R. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 12491-6. PMID: 11606717

3. Nykjaer, A. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13895-900. PMID: 11717447

4. Fyfe, JC. et al. (2004) Blood 103, 1573-9. PMID: 14576052

5. Kozyraki, R. et al. (1998) Blood 91, 3593-600. PMID: 9572993

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CUBN polyclonal antibody at 1ug/mL on recombinant CUBN (Catalog No. HT153012).

References

Datasheet

Anti-CUBN Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CUBN Polyclonal Antibody (HT153014)

Overview

Images

References

Datasheet

Quick access

Contact Us