Anti-Human RECQL4 Polyclonal Antibody (HF763014)

Name: Anti-Human RECQL4 Polyclonal Antibody
Brand: abinScience
SKU: HF763014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HF763014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RECQL4 (Gly556-Pro820).

Target

ATP-dependent DNA helicase Q4, RTS, RECQL4, RECQ4, DNA helicase, RecQ-like type 4, RecQ4, RecQ protein-like 4

Purification

Purified by antigen affinity column.

Accession

O94761

RRID

Anti-Human RECQL4 Polyclonal Antibody (abinScience Cat# HF763014, RRID:AB_3723133)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATP-dependent DNA helicase Q4 (RECQL4) is a ~133 kDa protein. An ATP-dependent DNA helicase which unwinds dsDNA with a 3'-overhang in a 3'-5' direction. Does not unwind more than 18 bp of dsDNA. May modulate chromosome segregation. The N-terminal domain (residues 1-54) binds DNA Y-shaped DNA better than ss- or dsDNA. The core helicase domain binds ssDNA.

1. Kaiser, S. et al. (2017) Nature communications 8, 15907. PMID: 28653661

2. Ohlenschläger, O. et al. (2012) Nucleic acids research 40, 8309-24. PMID: 22730300

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human RECQL4 polyclonal antibody at 1ug/mL on recombinant Human RECQL4 (Catalog No. HF763012).

References

Datasheet

Anti-Human RECQL4 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RECQL4 Polyclonal Antibody (HF763014)

Overview

Images

References

Datasheet

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