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Anti-TPM3 Polyclonal Antibody (HY532014)

Anti-TPM3 Polyclonal Antibody
Anti-TPM3 Polyclonal Antibody
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  • 50ug
  • 100ug
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  • Datasheet

Overview
Catalog No.HY532014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TPM3 (Met1-Ile285).
Target Gamma-tropomyosin, Tropomyosin-5, Tropomyosin alpha-3 chain, hTM5, Tropomyosin-3, TPM3
Purification Purified by antigen affinity column.
Accession P06753
RRID Anti-TPM3 Polyclonal Antibody (abinScience Cat# HY532014, RRID:AB_3723150)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tropomyosin alpha-3 chain (TPM3) is a ~32 kDa protein. Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Note For research use only.
Images
  • Anti-TPM3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TPM3 polyclonal antibody at 1ug/mL on recombinant TPM3 (Catalog No. HY532012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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