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Anti-Human UNC13D Polyclonal Antibody (HP122014)

Anti-Human UNC13D Polyclonal Antibody
Anti-Human UNC13D Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
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  • Datasheet

Overview
Catalog No.HP122014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human UNC13D (Ser888-Pro1090).
Target Munc13-4, Protein unc-13 homolog D, UNC13D
Purification Purified by antigen affinity column.
Accession Q70J99
RRID Anti-Human UNC13D Polyclonal Antibody (abinScience Cat# HP122014, RRID:AB_3723352)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein unc-13 homolog D (UNC13D) is a ~123 kDa protein. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.

Note For research use only.
Images
  • Anti-Human UNC13D Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Human UNC13D polyclonal antibody at 1ug/mL on recombinant Human UNC13D (Catalog No. HP122012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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