Anti-HMPV Phosphoprotein/P Polyclonal Antibody (VK739014)

Name: Anti-HMPV Phosphoprotein/P Polyclonal Antibody
Brand: abinScience
SKU: VK739014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK739014

Species reactivity

Human metapneumovirus (strain CAN97-83) (HMPV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HMPV Phosphoprotein/P (Met1-Met294).

Target

Phosphoprotein, Protein P, P

Purification

Purified by antigen affinity column.

Accession

Q8B9Q8

RRID

Anti-HMPV Phosphoprotein/P Polyclonal Antibody (abinScience Cat# VK739014, RRID:AB_3723653)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphoprotein is a ~32 kDa protein. Plays critical roles in regulating RNA replication and transcription through its interactions with multiple proteins. Tethers the RNA-directed RNA polymerase L to the nucleoprotein-RNA complex. Recruits the M2-1 protein, a processivity factor that is required for efficient transcription of viral RNA. Acts as a chaperone for neo-synthesized nucleoprotein by forming an N-P complex that preserves N in a monomeric and RNA-free state and prevents the association of nascent N with host cell RNAs. Recruits the host phosphatase PP1 to inclusion bodies to regulate viral transcription.

1. Pan, J. et al. (2020) Nature 577, 275-279. PMID: 31698413

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HMPV Phosphoprotein/P polyclonal antibody at 1ug/mL on recombinant HMPV Phosphoprotein/P (Catalog No. VK739012).

References

Datasheet

Anti-HMPV Phosphoprotein/P Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HMPV Phosphoprotein/P Polyclonal Antibody (VK739014)

Overview

Images

References

Datasheet

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