Anti-PEDV Spike glycoprotein (RBD) Polyclonal Antibody (VK564014)

Name: Anti-PEDV Spike glycoprotein (RBD) Polyclonal Antibody
Brand: abinScience
SKU: VK564014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK564014

Species reactivity

Porcine epidemic diarrhea virus (strain CV777) (PEDV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant PEDV Spike glycoprotein (RBD) (Phe617-Val745).

Target

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, S

Purification

Purified by antigen affinity column.

Accession

Q91AV1

RRID

Anti-PEDV Spike glycoprotein (RBD) Polyclonal Antibody (abinScience Cat# VK564014, RRID:AB_3723714)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Spike glycoprotein (RBD) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. RBD is the therapeutic target of casirivimab/imdevimab (REGEN-COV).

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PEDV Spike glycoprotein (RBD) polyclonal antibody at 1ug/mL on recombinant PEDV Spike glycoprotein (RBD) (Catalog No. VK564062).

References

Datasheet