Anti-Human UVRAG Polyclonal Antibody (HA311014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HA311014

Description

Anti-Human UVRAG Polyclonal Antibody (HA311014) is a rabbit polyclonal antibody detecting UVRAG in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UVRAG (Arg41-Ala164).

Target

UV radiation resistance-associated gene protein, p63, UVRAG

Purification

Purified by antigen affinity column.

Accession

Q9P2Y5

RRID

Anti-Human UVRAG Polyclonal Antibody (abinScience Cat# HA311014, RRID:AB_3723846)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

UV radiation resistance-associated gene protein (UVRAG) is a ~78 kDa protein. Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P). During autophagy acts as a regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2.

1. Liang, C. et al. (2006) Nature cell biology 8, 688-99. PMID: 16799551

2. Liang, C. et al. (2008) Nature cell biology 10, 776-87. PMID: 18552835

3. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123

4. He, S. et al. (2013) Nature cell biology 15, 1206-1219. PMID: 24056303

5. Cheng, X. et al. (2017) Molecular cell 65, 1029-1043.e5. PMID: 28306502

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human UVRAG polyclonal antibody at 1ug/mL on recombinant Human UVRAG (Catalog No. HA311012).

References

Datasheet

Anti-Human UVRAG Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human UVRAG Polyclonal Antibody (HA311014)

Overview

Images

References

Datasheet

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