Anti-Mouse CD265/TNFRSF11A Polyclonal Antibody (MV008014)

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Datasheet

Overview

Catalog No.

MV008014

Description

Anti-Mouse CD265/TNFRSF11A Polyclonal Antibody (MV008014) is a rabbit polyclonal antibody detecting TNFRSF11A in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD265/TNFRSF11A (Thr36-Thr200).

Target

Osteoclast differentiation factor receptor, Receptor activator of NF-KB, TNFRSF11A, ODFR, Tumor necrosis factor receptor superfamily member 11A, CD265, RANK

Purification

Purified by antigen affinity column.

Accession

O35305

RRID

Anti-Mouse CD265/TNFRSF11A Polyclonal Antibody (abinScience Cat# MV008014, RRID:AB_3723870)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tumor necrosis factor receptor superfamily member 11A (CD265/TNFRSF11A) is a ~66 kDa protein. Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2. Involved in the regulation of interactions between T-cells and dendritic cells.

1. Liu, C. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 184, 6910-9. PMID: 20483727

2. Choi, HK. et al. (2013) Cell research 23, 524-36. PMID: 23478294

3. Nakagawa, N. et al. (1998) Biochemical and biophysical research communications 253, 395-400. PMID: 9878548

4. Anderson, DM. et al. (1997) Nature 390, 175-9. PMID: 9367155

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Mouse CD265/TNFRSF11A polyclonal antibody at 1ug/mL on recombinant Mouse CD265/TNFRSF11A (Catalog No. MV008012).

References

Datasheet

Anti-Mouse CD265/TNFRSF11A Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD265/TNFRSF11A Polyclonal Antibody (MV008014)

Overview

Images

References

Datasheet

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