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Anti-MYBPC3 Polyclonal Antibody (HC262014)

Anti-MYBPC3 Polyclonal Antibody
Anti-MYBPC3 Polyclonal Antibody
Price(USD): $
Spec:
  • 50ug
  • 100ug
Number:
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  • Datasheet

Overview
Catalog No.HC262014
Description
Anti-MYBPC3 Polyclonal Antibody (HC262014) is a rabbit polyclonal antibody detecting MYBPC3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MYBPC3 (Met1-Glu451).
Target MYBPC3, Myosin-binding protein C, cardiac-type, C-protein, cardiac muscle isoform, Cardiac MyBP-C
Purification Purified by antigen affinity column.
Accession Q14896
RRID Anti-MYBPC3 Polyclonal Antibody (abinScience Cat# HC262014, RRID:AB_3724129)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Myosin-binding protein C, cardiac-type (MYBPC3) is a ~140 kDa protein. Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Note For research use only.
Images
  • Anti-MYBPC3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MYBPC3 polyclonal antibody at 1ug/mL on recombinant MYBPC3 (Catalog No. HC262022).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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