Anti-Human TDGF1/CRIPTO Polyclonal Antibody (HY096014)

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HY096014

Description

Anti-Human TDGF1/CRIPTO Polyclonal Antibody (HY096014) is a rabbit polyclonal antibody detecting CRIPTO in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TDGF1/CRIPTO (Leu31-Thr172).

Target

Cripto-1 growth factor, Teratocarcinoma-derived growth factor 1, TDGF1, CRIPTO, Epidermal growth factor-like cripto protein CR1, CRGF

Purification

Purified by antigen affinity column.

Accession

P13385

RRID

Anti-Human TDGF1/CRIPTO Polyclonal Antibody (abinScience Cat# HY096014, RRID:AB_3724148)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein Cripto (TDGF1) is a ~21 kDa protein. GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

1. Lee, GH. et al. (2016) The Journal of cell biology 215, 705-718. PMID: 27881714

2. Bianco, C. et al. (2002) Molecular and cellular biology 22, 2586-97. PMID: 11909953

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human TDGF1/CRIPTO polyclonal antibody at 1ug/mL on recombinant Human TDGF1/CRIPTO (Catalog No. HY096012).

References

Datasheet