Anti-Human LSM8 Polyclonal Antibody (HF981014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HF981014

Description

Anti-Human LSM8 Polyclonal Antibody (HF981014) is a rabbit polyclonal antibody detecting LSM8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LSM8 (Thr2-His96).

Target

U6 snRNA-associated Sm-like protein LSm8, LSM8

Purification

Purified by antigen affinity column.

Accession

O95777

RRID

Anti-Human LSM8 Polyclonal Antibody (abinScience Cat# HF981014, RRID:AB_3724467)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

U6 snRNA-associated Sm-like protein LSm8 is a ~10 kDa protein. Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.

1. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

2. Achsel, T. et al. (1999) The EMBO journal 18, 5789-802. PMID: 10523320

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human LSM8 polyclonal antibody at 1ug/mL on recombinant Human LSM8 (Catalog No. HF981012).

References

Datasheet

Anti-Human LSM8 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human LSM8 Polyclonal Antibody (HF981014)

Overview

Images

References

Datasheet

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