Anti-EEEV Spike glycoprotein E1 Polyclonal Antibody (VK634044)

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50ug
100ug

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Overview
Images
References
Datasheet

Overview

Catalog No.

VK634044

Description

Anti-EEEV Spike glycoprotein E1 Polyclonal Antibody (VK634044) is a rabbit polyclonal antibody detecting Spike glycoprotein E1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Eastern equine encephalitis virus (EEEV) (Eastern equine encephalomyelitis virus)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant EEEV Spike glycoprotein E1 (Tyr799-Gly1215).

Target

Spike glycoprotein E1

Purification

Purified by antigen affinity column.

Accession

P08768

RRID

Anti-EEEV Spike glycoprotein E1 Polyclonal Antibody (abinScience Cat# VK634044, RRID:AB_3724964)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Structural polyprotein (Spike glycoprotein E1) is a ~137 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Adams, LJ. et al. (2024) Cell 187, 360-374.e19. PMID: 38176410

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EEEV Spike glycoprotein E1 polyclonal antibody at 1ug/mL on recombinant EEEV Spike glycoprotein E1 (Catalog No. VK634042).
Western blot

Western blot analysis was performed using anti-EEEV1-E1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EEEV Spike glycoprotein E1 (Catalog No: VK634042)