Anti-PEDV S1/Spike glycoprotein 1 Polyclonal Antibody (VK564054)

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Spec:

50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

VK564054

Description

Anti-PEDV S1/Spike glycoprotein 1 Polyclonal Antibody (VK564054) is a rabbit polyclonal antibody detecting Spike glycoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Porcine epidemic diarrhea virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant PEDV S1/Spike glycoprotein 1 (Gln21-Glu496).

Target

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, S, Porcine epidemic diarrhea virus, PEDV

Purification

Purified by antigen affinity column.

Accession

S0AP48

RRID

Anti-PEDV S1/Spike glycoprotein 1 Polyclonal Antibody (abinScience Cat# VK564054, RRID:AB_3725066)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Spike glycoprotein (S1/Spike glycoprotein 1) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with the host receptor, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PEDV S1/Spike glycoprotein 1 polyclonal antibody at 1ug/mL on recombinant PEDV S1/Spike glycoprotein 1 (Catalog No. VK564012).
Western blot

Western blot analysis was performed using anti-S1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant PEDV S1/Spike glycoprotein 1 (Catalog No: VK564012)